Part:BBa_K2967005

a biosignal amplifier

A bio-amplifier is used to amplify the concentration of certain substances in the cell growth environment, such as the concentration of iptg, the concentration of arabinose and so on. The principle of this amplifier is that the hrpL promoter activity can only activate and amplify the pathway downstream of the promoter under the action of the HrpR and HrpS protein complex HrpRS. The sequence of the protein complex HrpRS specifically acting on the hrpL promoter region has not been investigated for a while, so the hrpL promoter region in the amplifier is relatively long. In order to ensure that the transcription initiation is completed normally, it is recommended not to change the sequence at will. The amplifier contains four partial coding genes hrpR, hrpS, T7terminator, and PhrpL+rbs for a total of 2222 bp. The amplifier portion is constructed between the rbs and the characterization molecule (eg, pc-am, gfp is the characterization molecule) to amplify the signal before rbs.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 957
  Illegal XbaI site found at 2233
  Illegal SpeI site found at 980
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 980
  Illegal NotI site found at 1911
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1892
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 957
  Illegal XbaI site found at 2233
  Illegal SpeI site found at 980
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 957
  Illegal XbaI site found at 2233
  Illegal SpeI site found at 980
  Illegal NgoMIV site found at 534
  Illegal AgeI site found at 121
  Illegal AgeI site found at 127
  Illegal AgeI site found at 175
  Illegal AgeI site found at 741
  Illegal AgeI site found at 766
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1102
  Illegal SapI.rc site found at 1735